Jinghui Liang, Weikang Yang, Xiangyu Meng, Peng Chen and Hongwu Du Pages 504 - 508 ( 5 )
Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF-TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.
Autoantigen, Behcet's disease, HnRNP A2/B1, Protein Overexpression and Purification.
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, 30 Xueyuan Rd, Beijing 100083, China.