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Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria

[ Vol. 23 , Issue. 1 ]

Author(s):

Malgorzata Slocińska, Grzegorz Rosinski and Wieslawa Jarmuszkiewicz   Pages 63 - 68 ( 6 )

Abstract:


It has been evidenced that mitochondrial uncoupling protein 4 (UCP4) and ATP-regulated potassium channel (mKATP channel) of insect Gromphadorhina coqereliana mitochondria decrease superoxide anion production. We elucidated whether the two energy-dissipating systems work together on a modulation of superoxide level in cockroach mitochondria. Our data show that the simultaneous activation of UCP4 by palmitic acid and mKATP channel by pinacidil revealed a cumulative effect on weakening mitochondrial superoxide formation. The inhibition of UCP4 by GTP (and/or ATP) and mKATP channel by ATP elevated superoxide production. These results suggest a functional cooperation of both energy-dissipating systems in protection against oxidative stress in insects.

Keywords:

Insects, mitochondria, mitochondrial ATP-regulated channel (mKATP channel), superoxide production, uncoupling protein 4 (UCP4).

Affiliation:

Department of Animal Physiology and Development, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

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