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Artificial polyepitope HIV-1 immunogen containing mimotope of 2F5 epitope

[ Vol. 23 , Issue. 2 ]

Author(s):

Nadezhda S. Shcherbakova, Dmitry N. Shcherbakov, Anastasiya Yu. Bakulina, Larisa I. Karpenko, Alexander B. Ryzhikov and Alexander A. Ilyichev   Pages 159 - 168 ( 10 )

Abstract:


Constructing a vaccine against HIV-1, able to induce production of broadly neutralizing antibodies, is crucial.

We report here the selection and characterization of RDWSFDRWSLSEFWL peptide mimotope that binds specifically to bNAbs 2F5. The peptide mimotope was selected from 15-mer phage-displayed peptide library by using Mab 2F5 as the selecting agent. The most abundant RDWSFDRWSLSEFWL peptide was inserted into a carrier, an artificial polyepitope immunogen - TBI (T- and B-cell immunogen). TBI-2F5 polyepitope immunogen that includes the mimotope of 2F5 epitope was constructed. It was shown that sera of mice immunized with TBI-2F5 protein recognized TBI protein as well as RDWSFDRWSLSEFWL peptide.

The capacity of sera of immunized mice to neutralize HIV-1 was demonstrated using subtype B env-pseudoviruses of HIV-1 QH0692.42 and PVO.4.

Based on these results, we conclude that peptide mimotope of 2F5 epitope RDWSFDRWSLSEFWL can be an essential component for a successful HIV-vaccine.

Keywords:

Humoral response to HIV-1, mAb 2F5, peptide mimotope, Phage display, HIV-1, vaccine against HIV-1.

Affiliation:

SRC VB Vector, Koltsovo, the Novosibirsk region, Russia

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