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Functional Peptides from Laminin-1 Improve the Cell Adhesion Capacity of Recombinant Mussel Adhesive Protein

[ Vol. 24 , Issue. 4 ]

Author(s):

Kai Wang, Lina Ji and Zichun Hua   Pages 348 - 352 ( 5 )

Abstract:


Since cell adhesion is important for cell processes such as migration and proliferation, it is a crucial consideration in biomaterial design and development. Based on the fusion of mussel adhesive protein fp151 with laminin-1-originated functional peptides we designed fusion proteins (fLA4, fLG6 and fAG73) and explored their cell adhesion properties. In our study, cell adhesion analysis showed that protein fLG6 and fLA4 had a significantly higher cell adhesion property for A549 than fp151. Moreover, protein fAG73 also displayed a strong adhesion capacity for Hela cells. In conclusion, the incorporation of functional peptides with integrin and heparin/heparan sulphate binding capacity into mussel adhesive protein will promote the application of mussel adhesive protein as cell adhesion biomaterial.

Keywords:

Cell adhesion materials, functional peptides, heparin/heparan sulfate binding peptide, integrin binding peptides, mussel adhesive protein.

Affiliation:

The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023, The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023, The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023

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