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Purification and Characterization of 2S Albumin from Seeds of Wrightia tinctoria Exhibiting Antibacterial and DNase Activity

[ Vol. 24 , Issue. 4 ]

Author(s):

Anchal Sharma, Pramod Kumar, Pooja Kesari, Neetu, Madhusudhanarao Katiki, Manisha Mishra, Pradhyumna K. Singh, Bhola R. Gurjar, Ashwani K. Sharma, Shailly Tomar and Pravindra Kumar   Pages 368 - 378 ( 11 )

Abstract:


2S albumin is a low-molecular-weight seed storage protein belonging to the prolamin superfamily. In the present work a small 2S albumin (WTA) protein of ~16 kDa has been purified from the seeds of Wrightia tinctoria. The WTA is a heterodimer protein with a small subunit of ~5 kDa and a larger subunit of ~11 kDa bridged together through disulphide bonds. The protein exhibits deoxyribonucleases activity against closed circular pBR322 plasmid DNA and linear BL21 genomic DNA. The protein also showed antibacterial activity against Morexalla catarrhalis. CD studies indicate a high α-helical content in the protein. The conserved disulphide bonds in the protein suggest that the WTA is highly stable under high pH and temperature like other 2S albumin.

Keywords:

2S Albumin, seed storage protein, Wrightia tinctoria, deoxyribonucleases activity, b-cell epitope, IgE binding allergen.

Affiliation:

Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Genetics and Plant Molecular Biology, Council of Scientific and Industrial Research (CSIR)–National Botanical Research Institute, Lucknow, Genetics and Plant Molecular Biology, Council of Scientific and Industrial Research (CSIR)–National Botanical Research Institute, Lucknow, Department of Civil Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand-247667

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