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Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness

[ Vol. 24 , Issue. 10 ]

Author(s):

Magda Melissa Florez, Camila Indiani de Oliveira, Concepcion Puerta, Fanny Guzman, Martha Ayala, Gladis Montoya and Gabriela Delgado*   Pages 982 - 988 ( 7 )

Abstract:


Background: The serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential.

Objective: This study was thus aimed at evaluating synthetic peptides derived from Leishmania braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA

Methods: It was used 8 and 13 peptides derived from ribosomal proteins 25 and S5 respectively as antigens in order to detect IgG antibodies by ELISA in people with active MCL, Chagas disease (CH) and autoimmune disease (AID).

Results: 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21.7%, 13.04%, 20% and 20%, respectively) as well as having 95%, 100%, 100% and 82.5% specificity, respectively.

Conclusion: The study revealed the limited usefulness of the peptides being studied as a diagnostic tool in the conditions used here, because its low sensitivity, but it is worth highlighting that the use of peptides as antigen in the serodiagnosis of MCL may overcome the cross reaction presented with other antigens, thus avoiding false positives.

Keywords:

Mucocutaneous leishmaniasis, diagnosis, synthetic peptide, ribosomal protein, parasite, Leishmania braziliensis.

Affiliation:

Grupo de Investigacion en Inmunotoxicologia Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Farmacia, Carrera 30 # 45-01, Bogota, Laboratorio de Inmunoparasitologia, Centro de Pesquisas Goncalo Moniz, FIOCRUZ, Salvador Bahia. Rua Waldemar Falcao, 121, Candeal - Salvador/BA, Grupo de Investigacion en Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 # 40- 62, Bogota, Nucleo de Biolotecnologia Curauma, Pontificia Universidad Catolica de Valparaiso, Avenida Universidad 330, Curauma, Valparaiso, Instituto Nacional de Salud, Laboratorio de Parasitologia, Avenida Calle 26 #51-20, Bogota, Centro de Estudio de Enfermedades Autoinmunes, Universidad del Rosario, Carrera 26 # 63B - 51, Bogota, Grupo de Investigacion en Inmunotoxicologia Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Farmacia, Carrera 30 # 45-01, Bogota

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