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Effect of Laser Therapy on Defensins’ Gene Expression in TR146 Epithelial Cell Line

[ Vol. 25 , Issue. 4 ]

Author(s):

Luisa Zupin*, Katia Rupel, Giulia Ottaviani, Augusto Poropat, Roberto Di Lenarda, Matteo Biasotto and Sergio Crovella   Pages 326 - 329 ( 4 )

Abstract:


Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects.

Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3.

Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform.

Results and Conclusion: In this cellular model LT decreased mRNA defensins' expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β -defensins production immediately after treatment, while its efficacy decreases over time.

Keywords:

Innate immunity, β-defensins, gene expression, laser therapy, oral mucositis, real time PCR.

Affiliation:

Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste

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