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Identification of Moesin as a Novel Autoantigen in Patients with Sjögren’s Syndrome

[ Vol. 25 , Issue. 4 ]

Author(s):

Yixuan Zhang, Muhammad Hussain, Xiuqin Yang, Peng Chen, Chunhe Yang, Yiping Xun, Yaping Tian* and Hongwu Du*   Pages 350 - 355 ( 6 )

Abstract:


Background: SjOgren's Syndrome (SS) is a systemic and chronic autoimmune disorder that affects the exocrine glands with massive autoantibody production. Although the pathogenesis of the disorder is incompletely understood, but some studies have reported that anti-moesin antibodies have been detected in autoimmune diseases with which SS is closely associated. Here, we have investigated moesin's potential involvement in SS.

Objective: This study aims to verify whether moesin is a specific autoantigen involved in Chinese Hans SS patients.

Methods: First, recombinant human moesin was expressed and purified. Next, the protein was verified as antigen by Western blotting and immunoprecipitation. The positive protein band in the immunoprecipitation was identified by (MALDI-TOF/TOF). Finally, an optimized ELISA (Enzyme- Linked Immunosorbent Assay) kit was developed to measure the titer concentration of anti-moesin antibody-positive patients in a large cohort of clinical subjects.

Results: Univariate analysis revealed that the proportion of individuals positive for serum IgG against recombinant human moesin was 42 % in a group of Chinese Hans SS patients (21 of 50), 22 % in systemic lupus erythematosus patients and (11 of 50), compared to only 4 % in healthy controls (2 of 50).

Conclusion: An association between anti-moesin antibodies and SS manifestation have been found which may be considered a suspected serum biomarker for the development of SS.

Keywords:

Autoantibody, autoantigen, moesin, Sjögren’s syndrome, autoimmune disorder, anti-moesin antibodies.

Affiliation:

112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, Experimental Research Center China Academy of Traditional chinese Medicine, Beijing, 100700, 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083

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