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Adjuvant Activity of Bursal Pentapeptide-(III-V) in Mice Immunized with the H9N2 Avian Influenza Vaccine

[ Vol. 25 , Issue. 8 ]

Author(s):

Jiangfei Zhou, Cong Zhang, Zhixin Liu, Yongqing Liu, Kairui Cai, Tengfei Shen, Chengshui Liao* and Chen Wang*   Pages 757 - 766 ( 10 )

Abstract:


Background: Bursal Pentapeptide (BPP)-(III-V) are peptides isolated from the Bursa of Fabricius (BF).

Method: The peptides' adjuvant activities were evaluated in mice immunized with the H9N2 avian influenza vaccine.

Results: The results showed that BPP-IV did not promote humoral or cellular immune responses. BPP-III had no effect on antibody production, but increased populations of CD3+ and CD8+ T cells, CTL levels and proliferation activities of splenic T-lymphocytes. BPP-V increased the level of antibodies (HI, IgG and its subtype IgG1) and the cytokine IL-4, as well as the ratio of CD3+ T cells and its subsets CD4+ and CD8+ T cells, BPP-V also increased CTL level and promoted the proliferation activity of T- and B-lymphocyte. Furthermore, in animal challenge experiment, BPP-III and BPP-V reduced viral RNA copies and viral titers, and reduced inflammatory cell infiltration.

Conclusion: In summary, BPP-IV did not have an effect on specific immune responses, therefore it could not reduce pathological damage in mice lungs; BPP-III provided resistance to the antigen through the cell-mediated immune response; BPP-V enhanced both humoral and cellular immune responses, therefore provided protections against the H9N2 Avian Influenza Virus (AIV). Altogether, BPP-III and BPP-V could be adjuvant candidates for the H9N2 avian influenza vaccine.

Keywords:

Bursal pentapeptided-(III-V), adjuvants, humoral immune response, cellular immune response, immunoprotection test, avian influenza virus.

Affiliation:

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan

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