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Structural Insights on the Obscurin-Binding Domains in Titin

[ Vol. 25 , Issue. 11 ]

Author(s):

Allyn G. Letourneau and Nathan T. Wright*   Pages 973 - 979 ( 7 )

Abstract:


Introduction: The giant muscular proteins titin and obscurin bind to each other at the Zdisk during muscle development. This binding event is mediated through two domains from each protein: ZIg9/10 from titin and Ig58/59 from obscurin. This interaction helps stabilize and organize the sarcomere; ablation of this binding leads to muscular dystrophy.

Objective: Here we solve the high-resolution solution structure of titin ZIg10 and further delineate which sections of titin bind to obscurin.

Materials and Methods: Solution NMR, Circular Dichroism, and SEC-MALS were used to biophysically characterize the titin domains involved in this titin-obscurin interaction.

Results and Conclusion: We present the high-resolution solution structure of titin ZIg10. Additionally, we show that titin ZIg9 drives the titin-obscurin interaction, while ZIg10 does not actively participate in the titin-obscurin interaction but instead acts to stabilize ZIg9.

Keywords:

Distal muscular dystrophy, titin, obscurin, solution NMR, high-resolution structure, muscle development.

Affiliation:

Department of Chemistry and Biochemistry, James Madison University, Harrisonburg, VA, Department of Chemistry and Biochemistry, James Madison University, Harrisonburg, VA

Graphical Abstract:



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