Submit Manuscript  

Article Details


Effect of Acyl Homoserine Lactone on Recombinant Production of Human Insulin-like Growth Factor-1 in Batch Culture of Escherichia coli

[ Vol. 25 , Issue. 11 ]

Author(s):

Valiollah Babaeipour, Hossein Vahidi, Shamila Alikhani, Javad Ranjbari*, Abbas Alibakhshi and Maryam Tabarzad*   Pages 980 - 985 ( 6 )

Abstract:


Background: IGF-I as a human growth factor produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Up to now, E. coli expression system has been widely used as the host to produce rhIGF-1 with high yields. Acyl Homoserine Lactones (AHLs) are intercellular signaling molecules used in quorum sensing by Gram-negative bacteria. Quorum sensing is a cell density-dependent gene regulation process that allows bacterial cells to express specific genes only when signaling molecules reach the sufficient concentration.

Objective: For the first time, this study focuses on the N-hexanoyl-L- Homoserine Lactone (HHL) activity on increasing the cell growth and rh-IGF-1concentration in batch culture of E. coli.

Method: The maximum production of rhIGF-I was previously optimized in 32y culture medium at 32°C with 0.05 mM IPTG as inducer and 10 g/l glucose concentration. Under this condition, different amounts of HHL (0.001 µg/ml, 1 µg/ml, and 100µg/ml) were evaluated as an inducer for IGF-1 production.

Results: Generally, with increasing of HHL concentration, an increase in dry cell weight (2.45 mg/ml to 4.63 mg/ml) and IGF-I expression level (0.4 mg/ml to 0.77 mg/ml) was observed.

Conclusion: HHL or other types of AHLs can be considered as protein production inducer in bacterial expression systems through the quorum sensing pathways.

Keywords:

Insulin like growth factor 1, acyl homoserine lactone, batch culture, fermentation, origami strain, E. coli, quorum sensing.

Affiliation:

Department of Biotechnology, Malek Ashtar University of Technology, Tehran, Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Protein Technology Research Center, Shahid Behashti University of Medical Sciences, Tehran

Graphical Abstract:



Read Full-Text article