Reza Khodarahmi*, Mirzaee Farideh, Leila Hosseinzadeh, Mohammad Reza Ashrafi-Kooshk, Sajjad Esmaeili, Sirous Ghobadi and Mohammad Hosein Farzaei Pages 1 - 16 ( 16 )
Background: Curcumin is a natural polyphenolic compound with anti-cancer, antiinflammatory, and anti-oxidation properties. Low water solubility and rapid hydrolytic degradation are two challenges limiting use of curcumin. Objective: In this study, the roles of the native/modified forms of Bovine Serum Albumin (BSA), β-lactoglobulin (β-lg) and casein, as food-grade biopolymers and also protein chemical modification, in stabilizing and on biological activity of curcumin were surveyed. Method: In this article, we used various spectroscopic as well as cell culture-based techniques along with calculation of thermodynamic parameters. Results: Investigation of curcumin stability indicated that curcumin binding to the native BSA and modified β-lg were stronger than those of the modified BSA and native β-lg, respectively and hence, the native BSA and modified β-lg could suppress water-mediated and light-mediated curcumin degradation, significantly. Moreover, in the presence of the native proteins (BSA and casein), curcumin revealed elevated in vitro anti-cancer activity against MCF-7 (human breast carcinoma cell line) and SKNMC (human neuroblastoma cell line). As well, curcumin, in the presence of the unmodified “BSA and β-lg”, was more potent to decrease ROS generation by hydrogen peroxide (H2O2) whereas it led to an inverse outcome in the presence of native casein. Overall, in the presence of the protein-bound curcumin, increased anti-cancer activity and decreased ROS generation by H2O2 in vitro were documented. Conclusion: It appears that “water exclusion” is major determinant factor for increased stability/ efficacy of the bound curcumin so that some protein-curcumin systems may provide novel tools to increase both food quality and the bioavailability of curcumin as health promoting agent.
Curcumin, Bovine serum albumin, Stability, Antioxidant activity, Oxidative stress
Kermanshah University of Medical Sciences - Medical Biology Research Center Kermanshah, Agricultural Faculty, Urmia University - Department of Food Science and Technology Urmia, Kermanshah University of Medical Sciences, - Pharmaceutical Sciences Research Center, School of Pharmacy Kermanshah, Medical Biology Research Center - Kermanshah University of Medical Sciences Kermanshah, Medical Biology Research Center - Kermanshah University of Medical Sciences Kermanshah, Razi University - Department of Biology, Faculty of Sciences Kermanshah, Medical Biology Research Center - Kermanshah University of Medical Sciences Kermanshah