Jianglong Peng, Hao Yin, Ying Zhou, Haoyuan Jia and Yubao Cui* Pages 184 - 191 ( 8 )
Background: The dust mite Dermatophagoides farinae is a common worldwide cause of indoor allergies induced by its proteins, including the mid-tier allergen Der f 7.
Objective: To identify conformational epitopes in Der f 7 using mimotope mapping and computational modelling.
Methods: Here, we used standard hybridoma technology to generate 3 new monoclonal antibodies against Der f 7 and performed mimotope mapping by probing a random peptide phage display library. Computational tools, including Minox and the DiscoTope-2.0 Server were used to assess the structure and potential position of antigenic residues within Der f 7.
Results: Thirteen mimotopes sharing the common sequence --XX[LST]P[-E][LI]MLPLR[-S]- were identified. Further, computationally-predicted conformational epitopes were found at residues 1-7, 10, 27, 76-81, 92, and 130-133 of Der f 7, and the key amino acids for these epitopes were deduced to be 2P, 3I, 10E, 27E, 78E, 79E, 81I, 130S, and 132E based on the common mimotope sequence.
Conclusion: We identified Der f 7 peptide mimotopes that may model binding sites for blocking antibodies. These may guide the development of immunotherapy for individuals with hypersensitivity to Der f 7.
House dust mite allergen, mimotopes, monoclonal antibody, random peptide library, Der f 7, indoor allergies.
School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou 571101, Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, Department of Pediatrics Laboratory, Wuxi Children's Hospital, Wuxi 214023, Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023