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Proliferative Effect of Tilapia Fish (Oreochromis niloticus) Lectin on BALB/c Mice Splenocytes

[ Vol. 26 , Issue. 12 ]

Author(s):

Cynarha Daysy Cardoso da Silva, Cristiane Moutinho Lagos de Melo*, Elba Verônica Matoso Maciel Carvalho, Mércia Andréa Lino da Silva, Rosiely Félix Bezerra, Athiê Jorge Guerra dos Santos, Valéria Rêgo Alves Pereira and Luana Cassandra Breitenbach Barroso Coelho   Pages 887 - 892 ( 6 )

Abstract:


Background: Lectins have been studied in recent years due to their immunomodulatory activities.

Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes.

Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis.

Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines.

Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.

Keywords:

Oreochromis niloticus, lectin, tilapia fish, cytokines, splenocytes, immunostimulation.

Affiliation:

Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Departamento de Antibióticos, Centro de Biociências, Recife, Pernambuco, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Departamento de Engenharia de Pesca, Universidade Federal Rural de Pernambuco/UFRPE, Pernambuco, Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães – CPqAM/FIOCRUZ, Pernambuco, Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco

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