Submit Manuscript  

Article Details


Investigation on the Interaction between Tetrakis (4-carboxylphenyl) Porphyrin and CopC by Spectroscopy and Docking Methods

Author(s):

Zhen Song*, Jin Liu, Wen Yuan, Ze Bai and Nvwa Gao   Pages 1 - 12 ( 12 )

Abstract:


Aims: The results may provide valuable information and thinking on the anti-cancer drug molecular design and the copper regulation mechanism.

Background: The copper chaperones CopC is regarded as a redox switch and possess barrel structure. Copper chaperone binding with small molecular may relate to the treatment of cancer. In the early-stage tumors, porphyrins were usually used to diagnosis.The results may provide valuable information and thinking on the anti-cancer drug molecular design and the copper regulation mechanism.

Objective: In this work, the interaction between the Tetrakis (4-carboxylphenyl) porphyrin (TCPP) and the copper chaperones CopC was studied.

Method: The interaction between CopC and TCPP were studied in details through fourier transform infrared spectroscopy (FTIR), fluorescence spectra, circular dichroism (CD), fluorescence lifetime and docking methods.

Result: FTIR and CD results indicated that the CopC conformation was changed by TCPP. The β-sheet content was reduced and the random coil content was increased. Moreover, fluorescence spectra data indicated that the combination ratio of TCPP-CopC was 1:1, and the inclusion constant is (5.88±0.12) × 105 M-1. In addition, Tb3+ was used to detect the interaction between CopC and TCPP as a probe. The result further verified that CopC can interact with TCPP. The thermodynamic parameters of interaction between CopC and TCPP (ΔH, ΔS) indicated that the force between CopC and TCPP was mainly hydrophobic interaction.

Conclusion: The results revealed that TCPP can form 1:1 complex with CopC, and the binding constant has been calculated to be (5.88±0.12) × 105 M-1. In addition, it was revealed that TCPP quench the fluorescence of CopC by the static quenching mechanism and the binding site n equals one. The formation of CopC-TCPP complex depended on the hydrophobic force and the distance between TCPP and tryptophan residue in CopC was 2.07 nm.

Keywords:

CopC, TCPP, Fluorescence, Copper, Dock, Interaction

Affiliation:

Taiyuan Normal University, Department of Chemistry, Jinzhong, Department of Urology, Hubei Provincial Corps Hospital, Chinese People’s Armed Police Forces, Wuhan, Taiyuan Normal University, Department of Chemistry, Jinzhong, Taiyuan Normal University, Department of Chemistry, Jinzhong, Taiyuan Normal University, Department of Chemistry, Jinzhong



Read Full-Text article