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Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics

Author(s):

Cleverson Diniz Texeira Freitas, Beatriz Caroline Nishi, Camila Tauane Monteiro do Nascimento, Maria Zelândia Rocha Silva, Eduardo Henrique Salviano Bezerra, Bruno Anderson Matias Rocha, Thalles Barbosa Grangeiro, João Pedro Brasil Oliveira, Pedro Filho Noronha Souza and Márcio Viana Ramos*   Pages 1 - 11 ( 11 )

Abstract:


Background: Osmotin-Like Proteins (OLPs) have been purified and characterized from different plant tissues, including latex fluids. Besides its defensive role, tobacco osmotin seems to induce adiponectin-like physiological effects, acting as an agonist. However, molecular information about this agonistic effect on adiponectin receptors has been poorly exploited and other osmotins have not been investigated yet.

Objective and Methods: The present study involved the characterization of three OLPs from Plumeria rubra latex and molecular docking studies to evaluate the interaction between them and adiponectin receptors (AdipoR1 and AdipoR2).

Results: P. rubra Osmotin-Like Proteins (PrOLPs) exhibited molecular masses from 21 to 25 kDa and isoelectric points ranging from 4.4 to 7.7. The proteins have 16 cysteine residues, which are involved in eight disulfide bonds, conserved in the same positions as other plant OLPs. The three-dimensional (3D) models exhibited the three typical domains of OLPs, and molecular docking analysis showed that two PrOLP peptides interacted with two adiponectin receptors similarly to tobacco osmotin peptide.

Conclusion: As observed for tobacco osmotin, the latex osmotins of P. rubra exhibited compatible interactions with adiponectin receptors. Therefore, these plant defense proteins (without known counterparts in humans) are potential tools to study modulation of glucose metabolism in type II diabetes, where adiponectin plays a pivotal role in homeostasis.

Keywords:

AdipoR1, AdipoR2, latex, molecular modeling, phylogeny of proteins, thaumatin

Affiliation:

Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal de Ceará, Departamento de Biologia, Fortaleza, Ceará, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900, Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Bloco 907, Campus do Pici. Fortaleza, Ceará, CEP 60440-900



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