Mohd Saeed, Vikas Kushvaha, Syed Mohd Faisal, Richa Verma, Irfan Ahmad, Huma Mustafa, Magdah Ganash, Mohammad Amjad Kamal and Ghulam Md Ashraf* Pages 1 - 10 ( 10 )
Background: Lymphatic filariasis (LF) is one of the incapacitating and mosquito-borne sicknesses that on progression may prompt a few recognizable types of clutters like extreme lymphedema, hydrocele, and elephantiasis
Methods: Antigenic preparations of B. malayi adult (BmA), S. cervi adult parasites and microfilariae (mf) total parasite extract were used to analyze the serological reactivity profile with human infectious sera collected from endemic areas of bancroftian filariasis by performing Western blot and ELISA analysis. Sera from healthy human subjects were also included in the study to determine the variation incurred in the reactivity due to the filariasis infection. Gel-electrophoresis analysis of the crude-extract of BmA revealed seven protein bands while more than ten bands in S. cervi.
Results: This represents the clear variation in protein patterns among the crude-antigens. ELISA results showed highest prevalence of IgG, IgM and IgG4 antibodies against all antigen preparations when recorded among microfilaraemic chronic infected patients. In both the antigenic preparations, the positive reactions were in the order of microfilaraemic>endemic normal>chronic>acute>non-endemic normal subjects. All sera of Mf+ patients were uniformly positive, while sera of both chronic and endemic normal subjects showed less reactivity.
Conclusion: In the present study, we endeavoured to establish the extent of cross-reactivity of antigens derived from animal filarial parasites such as B. malayi and S. cervi with W. bancrofti filariasis sera of human patients. Besides, we further analyzed antibody-isotype profile of IgG, IgG4 and IgM in various human infection sera of bancroftian filarial subjects reactive to heterologous parasite antigens derived from adult worms of S. cervi from bovine and B. malayi.
Lymphatic filariasis, Elephantiasis, Serological reactivity, heterologous parasite antigens, lymphedema, hydrocele
Department of Biology, College of Sciences University of Hail, Hail, Department of Zoology, Panjab University, Chandigarh, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Department of Biological Sciences, Alabama State University, Montgomery, AL, Department of Biological Sciences, Alabama State University, Montgomery, AL, Council of Science and Technology, Lucknow, UP, Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, King Fahd Medical Research Center, King Abdulaziz University, Jeddah