Karla Alejandra García-Borjas, Ivonne Ceballos-Olvera, Sarahí Luna-Castro* and Yosahandy Peña-Avelino Pages 101 - 107 ( 7 )
Background: Bovine Lactoferrin (bLf) has been reported as antimicrobial, antiviral, immunomodulatory and anticancer protein. Escherichia coli and Listeria spp. are food-borne bacteria that can produce illness in human being and mammals, the emergent antimicrobial drug resistance has been reported in these pathogens.Objective: The aim for this study was to evaluate the bLf effect on in vitro biofilm production and the synergic effect of antibiotics on E. coli and Listeria isolates. Methods: E. coli and Listeria specimens were isolated from bovine carcasses and slaughterhouses surfaces, respectively. Biofilm formation was analyzed with or without bLf, incubated for 48 h and spectrophotometry, cell viability was analyzed by colony-forming unit (CFU) and the synergistic effect of bLf with ampicillin, oxytetracycline, and streptomycin was evaluated through the fractional concentration index (FCI). Results: Our results show that a low bLf concentration (0.8 μM) can diminish the in vitro biofilm production in Listeria isolates; also improves the in vitro oxytetracycline and streptomycin activity against E. coli, and ampicillin activity against Listeria isolates. Conclusion: bLf can affect the biofilm production in Listeria isolates from slaughterhouses surfaces and shown synergic effect with ampicillin. Also has a synergic effect with oxytetracycline and streptomycin against E. coli isolates from bovine carcasses.
Bovine Lactoferrin, Escherichia coli, Listeria, biofilm production, synergy, antibiotics.
Faculty of Veterinary Medicine, Autonomous University of Tamaulipas, Victoria, Institute of Veterinary Science Research, Autonomous University of Baja California, Mexicali, Masters in Veterinary Science, Institute of Veterinary Science Research, Autonomous University of Baja California, Mexicali, Institute of Veterinary Science Research, Autonomous University of Baja California, Mexicali