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Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields

Author(s):

Farzaneh Sadeghzadeh, Amir Arsalan Entezari, Kiana Behzadian, Kimia Habibi, Zeinab Amiri-Tehranizadeh, Ahmad Asoodeh, Mohammad Reza Saberi and Jamshidkhan Chamani*   Pages 1 - 15 ( 15 )

Abstract:


Background: Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues.

Objectives: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different-frequency electromagnetic fields (EMF).

Methods: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF.

Result: The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF.

Conclusion: It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.

Keywords:

Drug-protein complexes, ACE inhibitory peptide, human hemoglobin, electromagnetic field, fluorescence spectroscopy, circular dichroism.

Affiliation:

Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University Mashhad, Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University Mashhad, Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University Mashhad, Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University Mashhad, Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University Mashhad



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