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Potential Application of CRISPR/Cas9 System to Engineer Abiotic Stress Tolerance in Plants

Author(s):

Temoor Ahmed, Muhammad Shahid*, Muhammad Noman, Sher Muhammad, Muhammad Tahir ul Qamar, Md. Arshad Ali, Awais Maqsood, Rahila Hafeez, Solabomi Olaitan Ogunyemi and Bin Li   Pages 1 - 17 ( 17 )

Abstract:


Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to develop crop varieties with improved tolerance against abiotic stresses. This review is centered on the biology and potential application of the CRISPR/Cas9 system to improve abiotic stress tolerance in plants. Furthermore, this review highlighted the recent advancements of CRISPR/Cas9-mediated genome editing for sustainable agriculture.

Keywords:

CRISPR/Cas9, abiotic stresses, genome editing, crop improvement, transcription, sgRNA.

Affiliation:

State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, , Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, , State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, , Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, , State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning-530004, Guangxi Province, , State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, , Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, , State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, , Department of Crop Protection, Federal University of Agriculture Abeokuta, Abeokuta, , State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou



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