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Oral angiotensin-(1–7) peptide modulates intestinal microbiota improving metabolic profile in obese mice

Author(s):

Amanda S Machado, Janaína R Oliveira, Deborah de F Lelis, Victor Hugo D Guimarães, Alfredo M B de Paula, André L S Guimarães, Igor V Brandi, Bruna Mara A de Carvalho, Diego Vicente da Costa, Cláudia Regina Vieira, Ulisses Alves Pereira, Theles de Oliveira Costa, João M O Andrade, Robson A S dos Santos and Sérgio H S Santos*  

Abstract:


Background: Obesity is a serious health problem which dysregulate Renin-Angiotensin System and intestinal microbiota.

Objective: The present study aimed to evaluate the Angiotensin-(1–7) [ANG-(1–7)] oral formulation effects on obese mice intestinal microbiota.

Methods: Mice were divided into four groups: obese and non-obese treated with ANG-(1–7) and obese and non-obese without ANG-(1–7) during four weeks.

Results: We observed a significant decrease in the fasting plasma glucose, total cholesterol, triglycerides, and Low-density lipoprotein levels and increased High-density lipoprotein in animals treated with ANG-(1–7). The histological analysis showed intestinal villi height reduction in mice treated with ANG-(1–7). Additionally, increased Bacteroidetes and decreased Firmicutes (increased Bacteroidetes/Firmicutes ratio) and Enterobacter cloacae populations were observed in the High-Fat Diet + ANG-(1–7) group. Receptor toll-like 4 (TLR4) intestinal mRNA expression was reduced in the HFD+ ANG-(1–7) group. Finally, the intestinal expression of the neutral amino acid transporter (B0AT1) was increased in animals treated with ANG-(1–7), indicating a possible mechanism associated with tryptophan uptake.

Conclusion: The results of the present study suggest for the first time an interaction between oral ANG-(1–7) and intestinal microbiota modulation.

Keywords:

Gut microbiota, metabolism, renin-angiotensin system, metabolic endotoxemia, small intestine, ACE2

Affiliation:

Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais, Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais, Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais, Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais, Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais, Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Laboratory of Health Science, Postgraduate Program in Health Sciences, Montes Claros, Minas Gerais



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