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Oral Treatment with Angiotensin-(1-7) Attenuates the Kidney Injury Induced by Gentamicin in Wistar Rats

Author(s):

Lílian Fernanda Pacheco*, Carlos Henrique de Castro, João Batista Rodrigues Dutra, Ruy de Souza Lino Junior, Patrícia Maria Ferreira, Robson Augusto Souza dos Santos and Cirano José Ulhoa   Pages 1 - 9 ( 9 )

Abstract:


Background: Acute Kidney Injury (AKI), a common disease of the urinary system, can be induced by high doses of gentamicin (GM). The renin-angiotensin system exerts a key role in the progression of the AKI since elevated intrarenal levels of Ang II, and ACE activity is found in this condition. However, it is unknown whether oral administration of angiotensin (Ang)-(1-7), a heptapeptide that evokes opposite effects of Ang II, may attenuate the renal injuries induced by gentamicin.

Objectives: To evaluate the effects of Ang-(1-7) on GM-induced renal dysfunction in rats.

Methods: AKI was induced by subcutaneous administration of GM (80 mg/Kg) for 5 days. Simultaneously, Ang-(1-7) included in hydroxypropyl β-cyclodextrin (HPβCD) was administered by gavage [46 μg/kg HPβCD + 30 μg/kg Ang-(1-7)]. At the end of the treatment period (sixth day), the rats were housed in metabolic cages for renal function evaluation. Thereafter, blood and kidney samples were collected.

Results: Ang-(1-7) attenuated the increase of the plasmatic creatinine and proteinuria caused by GM but did not change the glomerular filtration rate nor tubular necrosis. Ang-(1-7) attenuated the increased urinary flow and the fractional excretion of H2O and potassium observed in GM rats but intensified the elevated excretion of sodium in these animals. Morphological analysis showed that Ang-(1-7) also reduced the tubular vacuolization in kidneys from GM rats.

Conclusion: Ang-(1-7) promotes selective beneficial effects in renal injuries induced by GM.

Keywords:

Hidroxipropil-Beta-cyclodextrin, HPβCD, kidney function, nephrotoxicity, renin-angiotensin system, renoprotective effect.

Affiliation:

Superior School of Physical Education and Physiotherapy of the State of Goiás, State University of Goiás (UEG), Goiânia-Goiás, Department of Physiology, Institute of Biological Sciences, Federal University of Goiás (UFG), Goiânia-GO, Department of Physiology, Institute of Biological Sciences, Federal University of Goiás (UFG), Goiânia-GO, Department of Biosciences and Technology, Tropical Pathology and Public Health Institute, Federal University of Goiás (UFG), Goiânia, Department of Physiology, Institute of Biological Sciences, Federal University of Goiás (UFG), Goiânia-GO, Department of Physiology and Biophysics, Institute of Biological Sciences. Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Department of Biochemistry and Molecular Biology, Institute of Biological Sciences, Federal University of Goiás (UFG), Goiânia, Goiás



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