Jikui Song, John L. Markley, Jikui Song and John L. Markley Pages 265 - 268 ( 4 )
As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.
Affinity tag, docking, dynein, NMR spectroscopy, protein dynamics, protein-protein interactions
Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA., Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA.