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Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris

[ Vol. 15 , Issue. 4 ]

Author(s):

C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira and T. B. Grangeiro   Pages 327 - 332 ( 6 )

Abstract:


A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.

Keywords:

Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast

Affiliation:

Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Universidade Federal do Ceara -Campus do Pici, Av. Humberto Monte s/n CEP 60.455-970, Fortaleza,Ceara, Brazil.



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