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Characterization of Fluoroalcohols-Induced Intermediates of Mucor miehei Lipase at Low pH

[ Vol. 15 , Issue. 4 ]

Author(s):

Sadaf Fatima, Aseem Mishra, Priyankar Sen and Rizwan Hasan Khan   Pages 346 - 352 ( 7 )

Abstract:


We have previously characterized an acid-unfolded (UA) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with β-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. α-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.

Keywords:

Lipase, Mucor miehei, Molten Globule like state, circular dichroism, partially folded intermediate

Affiliation:

Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh - 202 002, India.



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