Submit Manuscript  

Article Details


Purification of a Chitin-Binding Protein from Moringa oleifera Seeds with Potential to Relieve Pain and Inflammation

[ Vol. 18 , Issue. 11 ]

Author(s):

Mirella Leite Pereira, Hermogenes David de Oliveira, Jose Tadeu Abreu de Oliveira, Juliana Menezes Gifoni, Raquel de Oliveira Rocha, Daniele de Oliveira Bezerra de Sousa and Ilka Maria Vasconcelos   Pages 1078 - 1085 ( 8 )

Abstract:


Moringa oleifera Lam. is a perennial multipurpose tree that has been successfully used in folk medicine to cure several inflammatory processes. The aim of this study was to purify and characterize a chitin-binding protein from Moringa oleifera seeds, named Mo-CBP4, and evaluate its antinociceptive and anti-inflammatory effects in vivo. The protein was purified by affinity chromatography on chitin followed by ion exchange chromatography. Acetic acid-induced abdominal constrictions assay was used for the antinociceptive and anti-inflammatory activity assessments. Mo-CBP4 is a glycoprotein (2.9% neutral carbohydrate) composed of two protein subunits with apparent molecular masses of 28 and 18 kDa (9 kDa in the presence of reducing agent). The intraperitoneal injection of Mo-CBP4 (3.5 and 10 mg/kg) into mice 30 min before acetic acid administration potently and significantly reduced the occurrence of abdominal writhing in a dose dependent manner by 44.7% and 100%, respectively. In addition, the oral administration of the protein (10 mg/kg) resulted in 18% and 52.8% reductions in abdominal writhing when given 30 and 60 min prior to acetic acid administration, respectively. Mo-CBP4, when administered by intraperitoneal route, also caused a significant and dose-dependent inhibition of peritoneal capillary permeability induced by acid acetic and significantly inhibited leukocyte accumulation in the peritoneal cavity. In conclusion, this pioneering study describes that the chitin-binding protein Mo-CBP4, from M. oleifera seeds, exhibits anti-inflammatory and antinociceptive properties and scientifically supports the use of this multipurpose tree in folk medicine.

Keywords:

Antinociceptive effect, anti-inflammatory activity, bioactivity, chitin-binding protein, Moringa oleifera, protein purification, Mo-CBP4, chromatography, Moringaceae family, aurantiamide acetate, ad libitum, CEPA, lyophilised, Polyacrylamide Gel Electrophoresis, Glycoprotein Detection KitAntinociceptive effect, anti-inflammatory activity, bioactivity, chitin-binding protein, Moringa oleifera, protein purification, Mo-CBP4, chromatography, Moringaceae family, aurantiamide acetate, ad libitum, CEPA, lyophilised, Polyacrylamide Gel Electrophoresis, Glycoprotein Detection Kit

Affiliation:

Universidade Federal do Ceara, Departamento de Bioquimica e Biologia Molecular, 60440-990, Fortaleza, CE, Brazil.



Read Full-Text article