Ana Maria Sampaio Assreuy, Nilson Vieira Pinto, Mario Rogerio Lima Mota, Ana Vaneska Passos Meireles, Joao Batista Cajazeiras, Camila Bezerra Nobre, Pedro Marcos Gomes Soares and Benildo Sousa Cavada Pages 1107 - 1111 ( 5 )
The vasorelaxant effect of the lectin of Pisum arvense (PAL) seeds was investigated in rat aorta. PAL (10-100 µ g/ml) was applied on aorta rings, with or without endothelium, pre-contracted with phenylephrine (Phe; 0.1 µ M). Participation of endothelium derived relaxant factors was evaluated incubating the tissue with indomethacin (10 µ M), L-nitro arginine methyl ester (L-NAME, 100 µ M) and tetraethylammonium (TEA, 5 mM) before addition of PAL. The role of the lectin domain was investigated by addition of PAL into tissue in presence of glucose (3× 10-5 M), or N-acetyl Dglucosamine (GlcNAc; 3× 10-4 M). The importance of extracellular calcium (Ca2+e) or interaction with muscarinic receptors in the relaxant effect was evaluated by addition of PAL into aorta rings containing calcium free solution (OCa) and atropine (1 µ M), respectively. PAL induced concentration-dependent relaxation in endothelized aorta (IC50 =58.38± 1.87 µ g/ml), which was reversed by L-NAME and glucose. The lectin effect was totally inhibited when the preparation was inserted in OCa, but not in presence of atropine. Summarizing, our data showed a relaxant effect of PAL in isolated rat aorta rings in presence of endothelium, suggestive of interaction between the lectin carbohydrate binding sites with specific receptors located in vascular endothelial cells leading to nitric oxide synthase activation. This effect seems to require Ca2+e but is independent on muscarinic receptors interaction.
Nitric oxide, Pisum arvense, plant lectin, relaxant effect, PAL, GlcNAc, Lectins, TEA, EGTA, ANOVA, Bonferroni's test, vasodilator effect, muscarinic receptors, EDHF, AMPc e GMPcNitric oxide, Pisum arvense, plant lectin, relaxant effect, PAL, GlcNAc, Lectins, TEA, EGTA, ANOVA, Bonferroni's test, vasodilator effect, muscarinic receptors, EDHF, AMPc e GMPc
Department of Morphology, School of Medicine, Federal University of Ceara (UFC), Fortaleza-CE, Brazil.