Sara Bagheri, Pooneh Rahimi*, Rouhollah Vahabpour, Fatemeh Motevalli, Mohammad Shayestehpour, Mohammad Reza Amiran and Shaghayegh Yazdani Pages 1179 - 1184 ( 6 )
Background: HCV E2 glycoprotein is one of the most attractive proteins for designing an effective vaccine. Deletion of hydrophobic carboxyl-terminal region of this protein is necessary for its secretion, especially when it is expressed in E-coli. In this study we expressed this protein in truncated form and evaluated its application in developing an ELISA test and induction of humoral response in immunized mice.
Objectives: The purpose of this study was expression of HCV truncated E2 protein from JFH1 strain in E-coli BL21(DE3) and evaluation of its antigenicity.
Methods: Truncated E2 region from HCV genotype 2a (JFH1) was amplified by PCR and cloned into a pET28a (+) vector and was used to transform the E-coli DH5α strain. The recombinant E2 protein was evaluated both in an ELISA test and induction of humoral immunity in mice.
Results: Truncated E2 protein was expressed in BL21(DE3). Its specific antibody was detected in serum samples from HCV infected patients. Also, it could elicit a significant humoral immunity in mice.
Conclusion: Truncated form of E2 protein which has been expressed in E-coli could be used as an effective antigen both in diagnostic tests such as ELISA and also, as a protein-based vaccine candidate.
HCV E2 glycoprotein, E. coli BL21(DE3), JFH1 strain, ELISA test, Protein-based vaccine, antigenicity.
MSc in Microbial Biotechnology, Biology Group, Payameh Nour University, Tehran, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Medical Lab Technology Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, School of Public Health, Tehran University of Medical Sciences, Tehran, Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, School of Public Health, Tehran University of Medical Sciences, Tehran