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A Simple Method for Constructing a Tagged Protein

[ Vol. 12 , Issue. 7 ]

Author(s):

C. Jo, J. Kim and S. A. Jo   Pages 631 - 633 ( 3 )

Abstract:


We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

Keywords:

gene fusion, recombinant protein, affinity tag, polymerase chain reaction (pcr), small ubiquitin-related modifier (sumo)

Affiliation:

Department of Biomedical Sciences and Biomedical Brain Research Center, National Institute ofHealth, 5 Nokbun-dong, Eunpyung-gu, Seoul [122-701], Korea;



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