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Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch

[ Vol. 12 , Issue. 7 ]

Author(s):

Chong Li, Wei Liu, Frank Bossmans, Rong-Huan Zhu, Jan Tytget and Da-Cheng Wang   Pages 635 - 638 ( 4 )

Abstract:


The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.

Keywords:

bmk it-ap, e. coli, recombinant expression, denaturation and renaturation

Affiliation:

Center for Structuraland Molecular Biology, Institute of Biophysics, Chinese Academyof Science, Beijing 100101, People's Republic of China;



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