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Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli

[ Vol. 12 , Issue. 7 ]

Author(s):

Xiaoping Wu, Xiaokun Li, Zhijian Su, Qing Zheng, Hua Xu, Sixian Wu, Ya Feng and Wen Zhao   Pages 659 - 661 ( 3 )

Abstract:


Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.

Keywords:

vasostatin, soluble expression, purification

Affiliation:

Biopharmaceutical Research&Development Center, Pharmacy College, Jinan University, Guangzhou510632, P. R. China;



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