David Bar-Or, Leonard T. Rael, Gregory W. Thomas and Jan P. Kraus Pages 271 - 273 ( 3 )
Copper was added to truncated, recombinant cystathionine β-synthase (CBS), and the enzyme activity was assessed by measuring the production of cystathionine. 10microM copper significantly decreased CBS activity by 50% while 25microM copper decreased CBS activity by 70%. This inhibition was negated when an analog of the N-terminus of human albumin, Asp-Ala-His-Lys (DAHK), a strong transition metal binding peptide, was added. The use of copper chelators could significantly reduce in vivo homocysteine levels.
cystathionine synthase, inhibition, copper, chelation, homocysteine
Department of Trauma Research and Trauma Services, Swedish Medical Center, 501 E. HampdenAvenue, Englewood, CO 80113, USA.