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Inhibitory Effect of Copper on Cystathionine β-Synthase Activity: Protective Effect of an Analog of the Human Albumin N-Terminus

[ Vol. 12 , Issue. 3 ]

Author(s):

David Bar-Or, Leonard T. Rael, Gregory W. Thomas and Jan P. Kraus   Pages 271 - 273 ( 3 )

Abstract:


Copper was added to truncated, recombinant cystathionine β-synthase (CBS), and the enzyme activity was assessed by measuring the production of cystathionine. 10microM copper significantly decreased CBS activity by 50% while 25microM copper decreased CBS activity by 70%. This inhibition was negated when an analog of the N-terminus of human albumin, Asp-Ala-His-Lys (DAHK), a strong transition metal binding peptide, was added. The use of copper chelators could significantly reduce in vivo homocysteine levels.

Keywords:

cystathionine synthase, inhibition, copper, chelation, homocysteine

Affiliation:

Department of Trauma Research and Trauma Services, Swedish Medical Center, 501 E. HampdenAvenue, Englewood, CO 80113, USA.



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