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One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System

[ Vol. 8 , Issue. 4 ]

Author(s):

Chen Li. Wei, Feng Ya. Ping, Zhou De He, Xu xue-Jun, Chen Jie, Wei Qiang and Chi Zhi-Qiang   Pages 265 - 272 ( 8 )

Abstract:


Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.

Keywords:

BACULOVIRUS, opioid receptor (MOR), Spodoptera frugiperda, Trichoplusia ni, Receptor binding assay

Affiliation:

Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Science, Chinese Academy ofSciences, Shanghai 200031, China



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