Chen Li. Wei, Feng Ya. Ping, Zhou De He, Xu xue-Jun, Chen Jie, Wei Qiang and Chi Zhi-Qiang Pages 265 - 272 ( 8 )
Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.
BACULOVIRUS, opioid receptor (MOR), Spodoptera frugiperda, Trichoplusia ni, Receptor binding assay
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Science, Chinese Academy ofSciences, Shanghai 200031, China