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Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra

[ Vol. 8 , Issue. 4 ]


Keith A. Oberg and Vladimir N. Uversky   Pages 297 - 302 ( 6 )


Secondary structure composition of two homologous proteins, alpha-fetoprotein and serum albumin, has been compared using circular dichroism and Fourier-transform infrared spectroscopy. CD-only analysis gives an accurate a-helical content for SA, but underestimates the predicted value for AFP. In contrast, FTIR-only analysis underestimates the helical content of SA. However, analysis of hybrid IR-CD spectra gives more accurate values for secondary structure composition for both AFP and SA and shows that the secondary structures of these proteins are the same.


Proteins Fetoprotein, Serum Albumin, Circular Dichroism, Human serum albumin (HSA)


Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA

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