Submit Manuscript  

Article Details


Secondary Structure of the Homologous Proteins, A A-Fetoprotein and Serum Albumin, from their Circular Dichroism and Infrared Spectra

[ Vol. 8 , Issue. 4 ]

Author(s):

Keith A. Oberg and Vladimir N. Uversky   Pages 297 - 302 ( 6 )

Abstract:


Secondary structure composition of two homologous proteins, alpha-fetoprotein and serum albumin, has been compared using circular dichroism and Fourier-transform infrared spectroscopy. CD-only analysis gives an accurate a-helical content for SA, but underestimates the predicted value for AFP. In contrast, FTIR-only analysis underestimates the helical content of SA. However, analysis of hybrid IR-CD spectra gives more accurate values for secondary structure composition for both AFP and SA and shows that the secondary structures of these proteins are the same.

Keywords:

Proteins Fetoprotein, Serum Albumin, Circular Dichroism, Human serum albumin (HSA)

Affiliation:

Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA



Read Full-Text article