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Crystallization and Preliminary X-Ray Crystallographic Studies of Human Autocrine Motility Factor

[ Vol. 8 , Issue. 4 ]


Hiroshi Uemura, Nobutada Tanaka, Tatsuyshi Funasaka, Arayo Haga, Hisamitsu Nagase, Avraham Raz and Kazuo T. Nakamura   Pages 317 - 322 ( 6 )


Human autocrine motility factor (hAMF), a tumor-secreted cytokine which stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 80.79 A, b = 107.1 A, and c = 270.9 A. There are two dimers per asymmetric unit. The crystals diffract to at least 2.0 A resolution and are suitable for X-ray structure analysis at high resolution.


Crystallization, Motility Factor, cytokine


School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, JAPAN

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