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Crystallization and X-Ray Data Analysis of the Extended Dna-Binding Domain of the E2 Bovine Papillomavirus Type 1 Protein

[ Vol. 8 , Issue. 4 ]

Author(s):

Joao a.r.g. Barbosa, Carlos Basilio Pinheiro, Adriana C. Alves, Jose R. Brandao neto, Leonardo G. Alonso, Gonzalo de Prat-Gay and Igor Polikarpov   Pages 323 - 326 ( 4 )

Abstract:


Hanging-drop vapour-diffusion method was used for the crystallization of the extended DNA-binding domain (residues 309-410) of the E2 protein from Bovine Papillomavirus Type 1. X-ray data collection at 2.0 A resolution was performed using synchrotron radiation. The crystal symmetry could be described by the space group P3121 and with unit cell parameters a = b = 55.3 A, c = 203.4 A. The protein structure was solved by molecular replacement.

Keywords:

DNA-binding domain, E2 Bovine PapillomaVirus type 1 protein

Affiliation:

Laboratorio Nacional de Luz Sincrotron, Caixa Postal 6192, CEP 13084-971, Campinas - SP, Brazil b Fundacion Campomar, Av. Patricias Argentinas 435 (1405), Buenos Aires, Argentina



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