Xiangming Fang, Li Peng, Zhinan Xu, Jinmin Wu and Peilin Cen Pages 31 - 37 ( 7 )
Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.
Human beta-defensin-2 (hBD-2), proinflammatory agents, anti-Gram-negative, transcription-polymerase chain reaction (RT-PCR), QIAprep Spin Minprep Kit, Chelating column, Coomassie brilliant blue G-250
The Central Laboratory of Sir Run Run Shaw Hospital, School of Medical Science, Zhejiang University, Hangzhou 310027, The People's Republic of China