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Cloning and Expression of Human Beta-defensin-2 Gene in Escherichia Coli

[ Vol. 9 , Issue. 1 ]

Author(s):

Xiangming Fang, Li Peng, Zhinan Xu, Jinmin Wu and Peilin Cen   Pages 31 - 37 ( 7 )

Abstract:


Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.

Keywords:

Human beta-defensin-2 (hBD-2), proinflammatory agents, anti-Gram-negative, transcription-polymerase chain reaction (RT-PCR), QIAprep Spin Minprep Kit, Chelating column, Coomassie brilliant blue G-250

Affiliation:

The Central Laboratory of Sir Run Run Shaw Hospital, School of Medical Science, Zhejiang University, Hangzhou 310027, The People's Republic of China



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