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Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

[ Vol. 9 , Issue. 1 ]

Author(s):

Beatriz Dos Santos Ferreira, Alan Trindade Branco, Karlla Salim de Queiroz and Goncalo Apolinario de Souza Filho   Pages 39 - 43 ( 5 )

Abstract:


A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Keywords:

isoelectric protein focusing (IEF), sodium dodecylsulfate (SDS)-PAGE, mercaptoethanol, Ampholytes solution, maize endosperm

Affiliation:

Laboratorio de Biotecnologia, Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, CEP 28015-620, Campos dos Goytacazes-RJ, Brazil



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