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Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile

[ Vol. 17 , Issue. 5 ]

Author(s):

Basir Ahmad, Zeyaul Islam, Ankita Varshney and Rizwan Hasan Khan   Pages 660 - 666 ( 7 )

Abstract:


The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichroism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hydrophobic clusters.

Keywords:

Acetonitrile, alkali denaturation, bovine serum fetuin, circular dichroism, trifluoroethanol

Affiliation:

Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh–202002, India.



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