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Ribosome Display and Selection of Human Anti-Placental Growth Factor scFv Derived from Ovarian Cancer Patients

[ Vol. 17 , Issue. 5 ]

Author(s):

Fang Li, Pingping Su, Chao Lin, Hong Li, Jiajing Cheng and Donglu Shi   Pages 585 - 590 ( 6 )

Abstract:


Ribosome display is a powerful cell-free technology to select a desired antibody together with its encoding mRNA. In this study, a human single-chain variable fragment (scFv) library was generated from the peripheral blood lymphocyte RNA of 10 ovarian cancer patients and then panned against bead-conjugated human PlGF, a protein that may contribute to the growth and metastasis of ovarian cancer. A selected scFv antibody was evaluated by Western blot and its affinity constant to the PlGF was determined by noncompetitive enzyme immunoassay. This study highlights the ribosomal display technology for the selection of human antibody from patient-derived gene pools.

Keywords:

Ribosome display, In vitro selection, Antibody engineering, PlGF, scFv, Ovarian cancer

Affiliation:

Department of Obstetrics and Gynecology, Affiliated Tenth Hospital, Tongji University, Shanghai, 200072, People's Republic of China; and The Institute for Advanced Materials and Nano Biomedicine, Tongji University, Shanghai, 200072, People's Republic of China.



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