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Functional Characterization of the In Vitro Folded Human Y1 Receptor in Lipid Environment

[ Vol. 17 , Issue. 5 ]

Author(s):

Susann Schimmer, Diana Lindner, Peter Schmidt, Annette G. Beck-Sickinger, Daniel Huster and Rainer Rudolph   Pages 605 - 609 ( 5 )

Abstract:


We describe the recombinant production of the human Y1 receptor from inclusion bodies of E. coli cultures. The in vitro refolding was carried out in the presence of lipids from bovine brain extracts. Y1 receptors in brain lipids compete for cellular receptors in competitive binding experiments.

Keywords:

GPCR, Y1 receptor, E. coli, in vitro folding, brain lipids

Affiliation:

Institute of Medical Physics and Biophysics, University of Leipzig, Germany.



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